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Drug Test - How Long Do Drugs Stay In Your System
Drug Detection Times - Drug Detection Window
How long do drugs stay in your system?
The length of time any drug stays in your system will vary. This is true for both the illicit drug and the prescribed drug. In large part, it depends on your physiological makeup and metabolism. These factors include your physical height, weight, your percentage of body fat, your age, current state of health and if you exercise (how often and how much). See the How Long Do Drugs Stay In Your System Chart below for more information on drug screen detection times.
For Alcohol Detection Times, see our "Breath Alcohol Calculator".
Home Drug Test - Will the test be positive?
The only way to know for sure is to use a drug test! Buy one today.
They are inexpensive, easy to use and provide instant results!
In determined how long drugs stay in your system, there is also the factor of whether or not you are undergoing any amount of stress at the time you take drugs. This is sometimes referred to as your "state of mind". Still other more important considerations include:
Your "frequency" of use. Have you used one time per day? Maybe 3 to 5 times per per day?
The "quantity" of drug you used each time (one or two "hits" or doses)
The "quality" (or potency) of the drug (was it pure, cut by 1/2, etc.)
The "length of time" (days, weeks, months) of your consistent drug use (prior to your drug test).
You must take into consideration variables including the overall body fluid balance and state of hydration. All of these factor help determine "how long" the drug is detectable in your system when your urine is analyzed or tested. Detectable levels depend upon the "cut off level" of the specific test being conducted. A detectable level means that the drug or its metabolite it as a high enough level that it shows up as a "positive" in a drug test.
The length of time that the presence of drugs of abuse in the body can be detected is an important factor in drug screening. The chart below outlines approximate duration times in human urine. For oral drug detection times see Saliva Drug Detection Window.
To know for sure if you or another person will SHOW UP CLEAN, perform a drug test!
Often drug users ask about detoxing or detoxification information and/or what is required to pass a drug test. For general information, see our, Detox-Pass A Drug Test page.
Drug Detection Times
in urine are expressed below in terms of lower and upper boundaries. Often we are asked, "How long does pot stay in my system?" or "On a drug test, how long does.... ?" The amount of time that a drug/metabolite remains detectable in urine can vary, depending on the following factors:
Amount and Frequency of Use: Single, isolated, small doses are generally detectable at the lower boundary. Chronic and long-term use typically result in detection periods near or at the upper boundary.
Metabolic Rate: Individuals with slower body metabolism are prone to longer drug detection periods.
Body Mass: In general, human metabolism slows with increased body mass, resulting in longer drug detection periods. In addition, THC (marijuana's active ingredient) and PCP are known to accumulate in fatty lipid tissue. Chronic users, physically inactive users, and individuals with a high percentage of body fat in relation to total body mass are prone to longer drug detection periods for THC and PCP.
Age: In general, human metabolism slows with age, resulting in longer drug detection periods.
Overall Health: In general, human metabolism slows during periods of deteriorating health, resulting in longer drug detection periods.
Drug Tolerance: Users typically metabolize a drug faster once a tolerance to the drug is established.
Urine pH: Urine pH can impact drug detection periods. Typically, highly acidic urine results in shorter drug detection periods.
Note: In a small percentage of cases, users may test positive longer than times shown - most notably in cases of long-term chronic abuse, in individuals with significant body mass and/or body fat, and in individuals with health related issues resulting in abnormally slow body metabolism. The "How Long Do Drugs Stay In Your System Chart" detailed below lists drug detection times for urine. For detailed information on saliva detection times, see How Long Do Drugs Show Up In Saliva
Drug Detection Times in Urine How Long Drugs Stay In Your System Chart
Drug / Drug Group |
Time Range |
Where To Buy Drug Test
Click on the link to find out more about this specific drug test or to purchase a home drug test kit. |
|---|---|---|
| Alcohol | 24 hours or less | Alcohol Drug Test |
| Amphetamines | 1 to 4 days | Amphetamines Drug Test |
| Barbiturates | Short-acting: 1 to 3 days | Barbiturates Drug Test |
| Long-acting (Barbital, Phenobarbital): 1 to 3 weeks | ||
| Benzodiazepines | Short-term Therapeutic Use: 1 to 3 days | Benzodiazepines Drug Test |
| Long-term / Chronic Use: 1 to 3 weeks | ||
|
benzoylecgonine metabolite |
1 to 5 days | Cocaine Drug Test |
| K2 - K3 - Spice | Spice Drug Test | |
| LSD | 1 to 2 days | LSD Drug Test |
| Marijuana (THC) | Casual Use: 1 to 7 days | Marijuana Drug Test |
| Long-Term / Chronic Use: 1 to 4 weeks | ||
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Note: THC, marijuana's primary active ingredient, is stored by the body in fatty lipid tissue. From there, it is slowly released into the bloodstream for up to several weeks - depending on the amount and frequency of use and the user's level of physical activity. In chronic and physically inactive users, THC may accumulate in fatty tissues faster than it can be eliminated. This accumulation leads to longer detection periods for these individuals. Also, users with a high percentage of body fat in relation to total body mass are prone to longer drug detection periods for marijuana. |
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| MDMA (Ecstasy) | 1 to 4 days | Ecstasy Drug Test - MDMA |
| Methadone | 1 to 4 days | Methadone Drug Test |
| Methamphetamines | 1 to 4 days | Meth Drug Test - METH |
| Nicotine (cotinine) | 2 to 3 days | Cotinine Test - Nicotine |
| Opiates | 1 to 5 days | Opiate Drug Test |
| Oxycodone includes Percocet, hydrocodone | 1 to 4 days | Oxycodone Drug Test |
| PCP (phencyclidine) | Casual Use: 1 to 7 days | PCP Drug Test |
| Long-Term / Chronic Use: 1 to 4 weeks | ||
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Note: PCP is stored by the body in fatty lipid tissue. From there, it is slowly released into the bloodstream for up to several weeks - depending on the amount and frequency of use and the user's level of physical activity. In chronic and physically inactive users, PCP may accumulate in fatty tissues faster than it can be eliminated. This accumulation leads to longer detection periods for these individuals. Also, users with a high percentage of body fat in relation to total body mass are prone to longer drug detection periods for PCP. |
Oral Salvia Drug Detection Times comparison of Urine verses Saliva Detection Times
How long do drugs stay in your system drug test - drug detection times for saliva, click the link above. This is a great comparison chart for drugs in system.
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Important Links:
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Drug Test FAQ - Frequently Asked Questions about drug tests and drug testing - Q & A for drug testing.
Glossary of Drug Terms - definitions applicable to the subjects of drug testing and pharmacology.
How To Use A Drug Test - Complete instructions on how to use various drug testing kits.
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Transmetron offers only the highest quality, instant drug test kits and supplies. Our tests are the same tests used by hospitals and clinics. Each test is FDA approved, easy to use and easy to read. We offer instant drug test dips, cassettes ( devices ), saliva drug tests, integrated drug test cups, breath alcohol and saliva alcohol tests. Our urine drug screen test kits can test for the following drugs: amphetamine ( AMP ), barbiturates, benzodiazepines ( BZO ), cocaine ( COC ), marijuana ( THC ), methadone ( MTD ), methamphetamine ( mAMP ) - meth, methylenedioxymethamphetamine ( MDMA ), morphine, opiate, opiates, phencyclidine ( PCP ), and tricyclic antidepressants ( TCA ). When you need to know, TRANSMETRON is the way to go!
For Detailed Information On Specific Drug Test Kits And Specifications
SEE BELOW.....
One Step Single/Multi-Drug Screen Test Panel
Package Insert for 1 to 10 Drug Screen Panel “Dip”
Instruction Sheet for testing of any combination of the following drugs:
AMP, BAR, BZO, COC,THC, MTD , mAMP, OPI, PCP AND TCA
A rapid, one step screening test for the simultaneous, qualitative detection of multiple drugs and drug metabolites in human urine. For healthcare professionals and professionals at point of care sites. For professional in vitro diagnostic use.
INTENDED USE
The One Step Multi-Drug Screen Test Panel is a lateral flow chromatographic immunoassay for the qualitative detection of multiple drugs and drug metabolites in urine at the following cut-off concentrations: 300 ng/mL Benzoylecgonine (Cocaine metabolite), 1,000 ng/mL Amphetamine, 1,000 ng/mL Methamphetamine, 50 ng/mL 11-nor-.9 -THC-9- COOH (THC), 2,000 ng/mL Opiate, 25 ng/mL Phencyclidine, in urine.
This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
SUMMARY
AMPHETAMINE (AMP)
Amphetamine is a Schedule II controlled substance available by prescription (Dexedrine®) and is also available on the illicit market. Amphetamines are a class of potent sympathomimetic agents with therapeutic applications. They are chemically related to the human body’s natural catecholamines: epinephrine and norepinephrine. Acute higher does lead to enhanced stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, and a sense of increased energy and power. Cardiovascular responses to Amphetamines include increased blood pressure and cardiac arrhythmias. More acute responses produce anxiety, paranoia, hallucinations, and psychotic behavior. The effects of Amphetamines generally last 2-4 hours following use, and the drug has a halflife of 4-24 hours in the body. About 30% of Amphetamines are excreted in the urine in unchanged form, with the remainder as hydroxylated and deaminated derivatives.
The AMP One Step Amphetamine Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Amphetamine in urine. The AMP One Step Amphetamine Test Strip yields a positive result when Amphetamines in urine exceed 1,000 ng/mL.
BARBITURATES (BAR)
Barbiturates are central nervous system depressants. They are used therapeutically as sedatives, hypnotics, and anticonvulsants. Barbiturates are almost always taken orally as
capsules or tablets. The effects resemble those of intoxication with alcohol. Chronic use of barbiturates leads to tolerance and physical dependence.
Short acting Barbiturates taken at 400mg/day for 2-3 months produces a clinically significant degree of physical dependence. Withdrawal symptoms experienced during periods of
drug abstinence can be severe enough to cause death.
Only a small amount (less than 5%) of most Barbiturates are excreted unaltered in urine. The approximate detection time limits for Barbiturates are:
| Short Acting (e.g. Secobarbital) | 100 mg PO (oral) | 4 – 5 days |
| Long Acting (e.g. Phenobarbital | 400 mg PO (oral) | 7 days1 |
The One Step Drug Screen Test yields a positive result when the Barbiturates in urine exceeds 300ng/ml.
BENZODIAZEPINES (BZO)
Benzodiazepines are medications that are frequently prescribed for symptomatic treatment of anxiety and sleep disorders. They produce their effects via specific receptors
involving a neurochemical called gamma aminobutyric acid (GABA). Because they are safer and more effective, Benzodiazepines have replaced barbiturates in the treatment of both
anxiety and insomnia. Benzodiazepines are also used as sedatives before some surgical and medical procedures, and for the treatment of seizure disorders and alcohol withdrawal.
Risk of physical dependence increases if Benzodiazepines are taken regularly (e.g., daily) for more than a few months, especially at higher than normal doses. Stopping abruptly
can bring on such symptoms trouble sleeping, gastrointestinal upset, feeling unwell, loss of appetite, sweating and trembling, weakness, anxiety and changes in perception.
Only trace amounts (less than 1%) of most Benzodiazepines are excreted unaltered in urine; most of the concentration in urine is conjugated drug. The detection period for the
Benzodiazepines in urine is 3 – 7 days.
The One Step Drug screen Test Card yields a positive result when the Benzodiazepines in urine exceeds 300 ng/ml.
COCAINE (COC)
Cocaine is a potent central nervous system (CNS) stimulant and a local anesthetic. Initially, it brings about extreme energy and restlessness while gradually resulting in
tremors, over-sensitivity and spasms. In large amounts, cocaine causes fever, unresponsiveness, and difficulty in breathing and unconsciousness.
Cocaine is often self-administered by nasal inhalation, intravenous injection and free-base smoking. It is excreted in the urine in a short time primarily as
Benzoylecgonine1,2. Benzoylecgonine, a major metabolite of cocaine, has a longer biological half-life (5-8 hours) than cocaine (0.5-1.5 hours), and can generally
be detected for 24-48 hours after cocaine exposure2.
The COC One Step Cocaine Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to
selectively detect elevated levels of cocaine metabolite in urine. The COC One Step Cocaine Test Strip yields a positive result when the cocaine metabolite in urine exceeds
300 ng/mL. This is the suggested screening cut-off for positive specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).
MARIJUANA (THC)
THC (.9--tetrahydrocannabinol) is the primary active ingredient in cannabinoids (marijuana). When smoked or orally administered, it produces euphoric effects. Users have
impaired short term memory and slowed learning. They may also experience transient episodes of confusion and anxiety. Long term relatively heavy use may be associated with
behavioral disorders. The peak effect of smoking marijuana occurs in 20-30 minutes and the duration is 90-120 minutes after one cigarette. Elevated levels of urinary metabolites
are found within hours of exposure and remain detectable for 3-10 days after smoking. The main metabolite excreted in the urine is 11-nor-.9-tetrahydrocannabinol-9-carboxylic
acid (.9-THC-COOH).
The THC One Step Marijuana Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to
selectively detect elevated levels of marijuana in urine. The THC One Step Marijuana Test Strip yields a positive result when the concentration of marijuana in urine exceeds
50 ng/mL. This is the suggested screening cut-off for positive specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).
METHADONE (MTD)
Methadone is a narcotic pain reliever for medium to severe pain. It is also used in the treatment of heroin (opiate dependence: Vicodin, Percocet, Morphine, etc.) addiction.
Oral Methadone is very different than IV Methadone. Oral Methadone is partially stored in the liver for late use. IV Methadone acts more like heroin. In most states you must go
to a pain clinic or a Methadone maintenance clinic to be prescribed Methadone.
Methadone is a long acting pain reliever producing effects that last from twelve to forth-eight hours. Ideally, Methadone frees the client from the pressures of obtaining
illegal heroin, from the dangers of injection and from the emotional roller coaster that most opiates produce. Methadone, if taken for long periods and at large doses, can lead
to a very long withdrawal period. The withdrawals from Methadone are more prolonged and troublesome than those provoked by heroin cessation, yet the substitution and phased
removal of methadone is an acceptable method of detoxification for patients and therapists.
The MTD One step Methadone test yields a positive result when Methadone in urine exceeds 300 ng/ml.
METHAMPHETAMINE (mAMP)
Methamphetamine is an addictive stimulant drug that strongly activates certain systems in the brain. Methamphetamine is closely related chemically to amphetamine, but the
central nervous system effects of Methamphetamine are greater. Methamphetamine is made in illegal laboratories and has a high potential for abuse and dependence. The drug can
be taken orally, injected, or inhaled. Acute higher does lead to enhanced stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, and a
sense of increased energy and power. Cardiovascular responses to Methamphetamine include increased blood pressure and cardiac arrhythmias. More acute responses produce anxiety,
paranoia, hallucinations, psychotic behavior, and eventually, depression and exhaustion.
The effects of Methamphetamine generally last 2-4 hours and the drug has a half-life of 9-24 hours in the body. Methamphetamine is excreted in the urine primarily as amphetamine
and oxidized and deaminated derivatives. However, 10-20% of Methamphetamine is excreted unchanged. Thus, the presence of the parent compound in the urine indicates
Methamphetamine use. Methamphetamine is generally detectable in the urine for 3-5 days, depending on urine pH level.
The mAMP One Step Methamphetamine Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody
to selectively detect elevated levels of Methamphetamine in urine. The mAMP One Step Methamphetamine Test Strip yields a positive result when the Methamphetamine in urine
exceeds 1,000 ng/mL.
OPIATE (300 ng/ml) (OPI 300 or MOP 300)
Opiate refers to any drug that is derived from the opium poppy, including the natural products, morphine and codeine, and the semi-synthetic drugs such as heroin. Opioid is
more general, referring to any drug that acts on the opioid receptor.
Opioid analgesics comprise a large group of substances which control pain by depressing the central nervous system. Large dose of morphine can produce higher tolerance levels,
physiological dependency in users, and may lead to substance abuse. Morphine is excreted unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine
is detectable in the urine for several days after an opiate dose. The OPI One Step Opiate Test Strip is a rapid urine screening test that can be performed without the use of an
instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of morphine in urine. The OPI One Step Opiate Test Strip yields a positive result when
the morphine in urine exceeds 300 ng/mL.
OPIATE (OPI) (2000 ng/ml)
Opiate refers to any drug that is derived from the opium poppy, including the natural products, morphine and codeine, and the semi-synthetic drugs such as heroin. Opioid is
more general, referring to any drug that acts on the opioid receptor.
Opioid analgesics comprise a large group of substances which control pain by depressing the central nervous system. Large dose of morphine can produce higher tolerance
levels, physiological dependency in users, and may lead to substance abuse. Morphine is excreted unmetabolized, and is also the major metabolic product of codeine and heroin.
Morphine is detectable in the urine for several days after an opiate dose.
The OPI One Step Opiate Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to
selectively detect elevated levels of morphine in urine. The OPI One Step Opiate Test Strip yields a positive result when the morphine in urine exceeds 2,000 ng/mL. This is the
suggested screening cut-off for positive specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).
PHENCYCLIDINE
Phencyclidine, also known as PCP or Angel Dust, is a hallucinogen that was first marketed as a surgical anesthetic in the 1950’s. It was removed from the market because
patients receiving it became delirious and experienced hallucinations.
Phencyclidine is used in powder, capsule, and tablet form. The powder is either snorted or smoked after mixing it with marijuana or vegetable matter. Phencyclidine is most
commonly administered by inhalation but can be used intravenously, intra-nasally, and orally. After low doses, the user thinks and acts swiftly and experiences mood swings
from euphoria to depression. Self-injurious behavior is one of the devastating effects of Phencyclidine.
PCP can be found in urine within 4 to 6 hours after use and will remain in urine for 7 to 14 days, depending on factors such as metabolic rate, user’s age, weight, activity,
and diet.5 Phencyclidine is excreted in the urine as an unchanged drug (4% to 19%) and conjugated metabolites (25% to 30%).
The PCP One Step Phencyclidine Test Strip is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to
selectively detect elevated levels of phencyclidine metabolite in urine. The PCP One Step Phencyclidine Test Strip yields a positive result when the phencyclidine
metabolite in urine exceeds 25 ng/mL. This is the suggested screening cut-off for positive specimens set by the Substance Abuse and Mental Health Services Administration
(SAMHSA, USA).
TRICYCLIC ANTIDEPRESSANT (TCA)
TCA (Tricyclic Antidepressants) are commonly used for the treatment of depressive disorders. TCA overdoses can result in profound central nervous system
depression, cardiotoxicity and anticholinergic effects. TCA overdose is the most common cause of death from prescription drugs. TCAs are taken orally or sometimes by
injection. TCAs are metabolized in the liver. Both TCAs and their metabolites are excreted in urine mostly in the form of metabolites for up to ten days.
The One Step Drug Screen Tests yields a positive result when the Tricyclic Antidepressant in urine exceeds 1,000 ng/ml.
PRINCIPLE
The One Step Multi-Drug Screen Test Panel is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen
compete against their respective drug conjugate for binding sites on their specific antibody.
During testing, a urine specimen migrates upward by capillary action. A drug, if present in the urine specimen below its cut-off concentration, will not saturate the binding
sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will show up in the test line region of the specific
drug strip. The presence of drug above the cut-off concentration will saturate all the binding sites of the antibody. Therefore, the colored line will not form in the test
line region.
A drug-positive urine specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative urine specimen
will generate a line in the test line region because of the absence of drug competition.
To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane
wicking has occurred.
REAGENTS
The test panel contains specific mouse monoclonal antibody, goat polyclonal antibody and drug protein conjugates.
PRECAUTIONS
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STORAGE AND STABILITY
Kit can be stored at room temperature or refrigerated at 2-30°C. The test panel is stable through the expiration date printed on the sealed pouch. The test panel must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
MATERIALS PROVIDED
Test panels
Package insert
MATERIALS REQUIRED BUT NOT PROVIDED
Specimen collection container
External controls
Timer
PREPARATION URINE ASSAY
The urine specimen must be collected in a clean and dry container. Urine collected at any time of the day may be used. Urine specimens exhibiting visible precipitates should be centrifuged, filtered, or allowed to settle to obtain a clear supernatant for testing.
SPECIMEN STORAGE
Urine specimens may be stored at 2-8°C for up to 48 hours prior to testing. For prolonged storage, specimens may be frozen and stored below -20°C. Frozen specimens should be thawed and mixed well before testing.
QUALITY CONTROL
A procedural control is included in the test. A colored line appearing in the control region (C) is considered an internal procedural control. It confirms
sufficient specimen volume, adequate membrane wicking and correct procedural technique.
Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test
procedure and to verify proper test performance.
LIMITATIONS
The One Step Multi-Drug Screen Test Panel provides only a qualitative, preliminary analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography and mass spectrometry (GC/MS) is the preferred confirmatory method.
There is a possibility that technical or procedural errors, as well as other interfering substances in the urine specimen may cause erroneous results.
Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results regardless of the analytical method used. If adulteration is suspected, the test should be repeated with another urine specimen.
A Positive result does not indicate level or intoxication, administration route or concentration in urine.
A Negative result may not necessarily indicate drug-free urine. Negative results can be obtained when drug is present but below the cut-off level of the test.
Test does not distinguish between drugs of abuse and certain medications.
PERFORMANCE CHARACTERISTICS - ACCURACY
A side-by-side comparison was conducted using The One Step Single Drug Test and commercially available drug rapid tests. Testing was performed on approximately 300 specimens previously collected from subjects presenting for Drug Screen Testing. Presumptive positive results were confirmed by GC/MS. The following compounds were quantified by GC/MS and contributed to the total amount of drugs found in presumptive positive urine samples tested.
| TEST Compounds Contributed to the Totals of GC/MS | TEST Compounds Contributed to the Totals of GC/MS |
|---|---|
| AMP Amphetamine | MTD Methadone |
| BAR Secobarbital, Butalbital, Phenobarbital, Pentobarbital | mAMP Methamphetamine |
| BZO Oxazepam, Nordiazepam, a-OH-Alprazolam, Desalklflurazepam | OPI Morphine, Codeine |
| COC Benzoylecgonine | PCP Phencyclidine |
| THC 11-nor-.9-tetrahydrocannabinol-carboxylic acid | TCA Nortriptyline |
The following results are tabulated from these clinical studies:
Forty (40) clinical samples for each drug were run using each of the One Step Single Drug tests by an untrained operator at a Professional Point of Care site. Based on GC/MS data, the operator obtained statistically similar Positive Agreement, Negative Agreement and Overall Agreement rates as trained Laboratory personnel.
*Note: TCA was based on HPLC data.
Precision
A study was conducted at three physician offices by untrained operators using three different lots of product to demonstrate the within run, between run and between operator precision. An identical panel of coded specimens, containing drugs at the concentration of ± 50% and ± 25% cut-off level, was labeled as a blind and tested at each site. The results are given below:
Analytical Sensitivity
A drug-free urine pool was spiked with drugs to the concentrations at ± 50% cut-off and ± 25% cut-off. The results are summarized below.
| Drug conc. (Cut-off range) | n | AMP | BAR | BZO | COC | THC | MTD | mAMP | OPI | PCP | TCA | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| - | + | - | + | - | + | - | + | - | + | - | + | - | + | - | + | - | + | - | + | ||
| 0% Cut-off | 30 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 1 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 |
| -50% Cut-off | 30 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 29 | 1 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 |
| -25% Cut-off | 30 | 30 | 0 | 27 | 3 | 26 | 4 | 30 | 0 | 12 | 1 | 24 | 6 | 30 | 0 | 30 | 0 | 19 | 11 | 22 | 8 |
| Cut-off | 30 | 18 | 12 | 22 | 8 | 12 | 18 | 4 | 26 | 1 | 29 | 21 | 9 | 18 | 12 | 30 | 17 | 16 | 14 | 12 | 18 |
| +25% Cut-off | 30 | 1 | 29 | 7 | 23 | 3 | 27 | 0 | 30 | 1 | 29 | 2 | 28 | 1 | 29 | 30 | 26 | 6 | 24 | 7 | 23 |
| +50% Cut-off | 30 | 0 | 30 | 2 | 28 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 |
Analytical Specificity
The following table lists the concentration of compounds (ng/mL) that are detected positive in urine by The One Step Multi-Drug Screen Test Panel at 5 minutes.
| AMPHETAMINE | ng/mL |
|---|---|
| D-Amphetamine | 1,000 |
| D,L-Amphetamine sulfate | 3,000 |
| L-Amphetamine | 50,000 |
| (±)3,4-Methylenedioxyamphetamine | 2,000 |
| Phentermine | 3,000 |
| Secobarbital | 300 |
| Amobarbital | 300 |
| Alphenol | 150 |
| Aprobarbital | 200 |
| Butalbital | 75 |
| Butethal | 2500 |
| Cyclopentobarbital | 100 |
| Pentobarbital | 600 |
| Phenobarbital | 300 |
| Benzodiazepines | |
| Oxazepam | 300 |
| Alprazolam | 196 |
| a-Hydroxyalprazolam | 1262 |
| Bromazepam | 1562 |
| Chlordiazepoxide | 1562 |
| Chlordiazepoxide HCI | 781 |
| Clobazam | 98 |
| Clonazepam | 781 |
| Clorazepate dipotassium | 195 |
| Delorazepam | 1562 |
| Desalkyflurazepam | 390 |
| Diazepam | 195 |
| Estazolam | 2500 |
| Flunitrazepam | 390 |
| ( + ) Lorazepam | 1562 |
| RS-Lorazepam glucuronide | 156 |
| Midazolam | 12500 |
| Nitrazepam | 98 |
| Norchlordiazepoxide | 195 |
| Nordiazepam | 390 |
| Temazepam | 98 |
| Triazolam | 2500 |
| COCAINE | ng/ml |
| Benzoylecgonine | 300 |
| Cocaine HCl | 780 |
| Cocaethylene | 12,500 |
| Ecgonine HCl | 32,000 |
| MARIJUANA (THC) | |
| 11-nor-.9 -THC-9 COOH | 50 |
| Cannabinol | 20,000 |
| 11-nor-.8-THC-9 COOH | 30 |
| .8 -THC | 15,000 |
| .9 -THC | 15,000 |
| Methadone | ng/mL |
|---|---|
| Methadone | 300 |
| Doxylamine | 50000 |
| METHAMPHETAMINE | |
| D-Methamphetamine | 1,000 |
| ñ-Hydroxymethamphetamine | 30,000 |
| L-Methamphetamine | 8,000 |
| (±)-3,4-Methylenedioxymethamphetamine | 2,000 |
| Mephentermine | 50,000 |
| OPIATES | ng/ml |
| Morphine | 2,000 |
| Codeine | 2,000 |
| Ethylmorphine | 5,000 |
| Hydrocodone | 12,500 |
| Hydromorphone | 5,000 |
| Levophanol | 75,000 |
| 6-Monoacetylmorphine | 5,000 |
| Morphine 3-â-D-glucuronide | 2,000 |
| Norcodeine | 12,500 |
| Normorphone | 50,000 |
| Oxycodone | 25,000 |
| Oxymorphone | 25,000 |
| Procaine | 150,000 |
| Thebaine | 100,000 |
| PCP | |
| Phencyclidine | 25 |
| 4-Hydroxyphencyclidine | 12,500 |
| TCA | |
| Nortriptyline | 1,000 |
| Nordoxepine | 1,000 |
| Trimipramine | 3,000 |
| Amitriptyline | 1,500 |
| Promazine | 1,500 |
| Desipramine | 200 |
| Imipramine | 400 |
| Clomipramine | 12,500 |
| Doxepin | 2,000 |
| Maprotiline | 2,000 |
| Promethazine | 25,000 |
Effect of Urinary Specific Gravity
Fifteen (15) urine samples of normal, high, and low specific gravity ranges (1.000-1.037) were spiked with drugs at 50% below and 50% above cut-off levels respectively. The Multi-Drug Screen Test was tested in duplicate using fifteen drug-free urine and spiked urine samples. The results demonstrate that varying ranges of urinary specific gravity does not affect the test results.
Effect of the Urinary pH
The pH of an aliquoted negative urine pool was adjusted to a pH range of 5 to 9 in 1 pH unit increments and spiked with drugs at 50% below and 50% above cut-off levels. The spiked, pH-adjusted urine was tested with The One Step Multi-Drug Screen Test Panel. The results demonstrate that varying ranges of pH does not interfere with the performance of the test.
Cross-Reactivity
A study was conducted to determine the cross-reactivity of the test with compounds in either drug-free urine or Cocaine, Amphetamine, Methamphetamine, Marijuana, Opiate or Phencyclidine positive urine. The following compounds show no cross-reactivity when tested with the One Step Multi-Drug Screen Test Panel at a concentration of 100 µg/mL.
Non Cross-Reacting Compounds
| Acetaminophen | Deoxycorticosterone | Loperamide | Promazine |
| Acetophenetidin | Dextromethorphan | Maprotiline | Promethazine |
| N-Acetylprocainamide | Diazepam | MDE | DL-Propranolol |
| Acetylsalicylic acid | Diclofenac | Meperidine | D-Propoxyphene |
| Aminopyrine | Diflunisal | Meprobamate | D-Pseudoephedrine |
| Amitryptyline | Digoxin | Methadone | Quinacrine |
| Amoxicillin | Diphenhydramine | Methoxyphenamine | Quinidine |
| Ampicillin | Doxylamine | Nalidixic acid | Quinine |
| L-Ascorbic acid | (-) -Ø-Ephedrine | Naloxone | Ranitidine |
| DL-Amphetamine sulfate | â-Estradiol | Naltrexone | Salicylic acid |
| Apomorphine | Estrone-3-sulfate | Naproxen | Serotonin |
| Aspartame | Ethyl-p-aminobenzoate | Niacinamide | Sulfamethazine |
| Atropine | [1R,2S] (-) Ephedrine | Nifedipine | Sulindac |
| Benzilic acid | (L) – Epinephrine | Norethindrone | Temazepam |
| Benzoic acid | Erythromycin | D-Norpropoxyphene | Tetracycline |
| Benzphetamine | Fenoprofen | Noscapine | Tetrahydrocortisone, 3-acetate |
| Bilirubin | Furosemide | DL-Octopamine | Tetrahydrocortisone 3- |
| (±) – Brompheniramine | Gentisic acid | Oxalic acid | (â-D-glucuronide) |
| Caffeine | Hemoglobin | Oxazepam | Tetrahydrozoline |
| Cannabidiol | Hydralazine | Oxolinic acid | Thiamine |
| Chloralhydrate | Hydrochlorothiazide | Oxymetazoline | Thioridazine |
| Chloramphenicol | Hydrocortisone | Papaverine | DL-Tyrosine |
| Chlorothiazide | O-Hydroxyhippuric acid | Penicillin-G | Tolbutamide |
| (±) – Chlorpheniramine | p-Hydroxyamphetamine | Pentazocine hydrochloride | Triamterene |
| Chlorpromazine | 3-Hydroxytyramine | Perphenazine | Trifluoperazine |
| Chlorquine | Ibuprofen | Phenelzine | Trimethoprim |
| Cholesterol | Imipramine | Trans-2-phenylcyclo-propylamine | Trimipramine |
| Clomipramine | Iproniazid | hydrochloride | Tryptamine |
| Clonidine | (±) – Isoproterenol | L-Phenylephrine | DL-Tryptophan |
| Cortisone | Isoxsuprine | â-Phenylethylamine | Tyramine |
| (-) Cotinine | Ketamine | Phenylpropanolamine | Uric acid |
| Creatinine | Ketoprofen | Prednisolone | Verapamil |
| Labetalol | Prednisone |
Transmetron
1725 South 1700 East
Salt Lake City, UT 84108
801-596-2709